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1. Neuroimmune-Communication in health and disease

The immune system and the brain are bidirectionally wired and it is of great importance to understand this interaction to dissect the pathogenesis of diseases and develop new therapies. The lecture introduces into the basics of neuroimmune-communication.


Realized by: Clinic and Polyclinic for Neurology - Department Neuroimmunology (Dr. Antje Vogelgesang)

Attendance: unlimited

Duration: 45 min.

Location: will follow



2. Flow cytometric analysis of murine immune cells

The Department of Immunology of the University Medicine Greifswald focusses on two main themes: The study of the immune response against S.aures infections as well as a sepsis model, which has been developed by the department itself. To answer questions concerning these themes, in vivo experiments with mice are inevitable.
During this workshop, we want to give the students the opportunity to get an insight into the need of work with laboratory animals. Therefore, immunologically important organs, such as thymus, spleen and lymph nodes are dissected off a mouse by an adviser. Immune cells are to be isolated out of these organs and analysed via flow cytometry. The flow cytometry is an important method for the visualization and distribution of cells in different organs and can give an overview of their activation state. In our practical part we are specifically going to deal with the distribution of T cells and their subpopulations in the above mentioned organs. The evaluation of the gained data will be realized by a software used in our laboratory.


Realized by: Institute for Immunology and Transfusion Medicine - Department Immunology (Dr. Grazyna Domanska, Julia Zeun)

Attendance: 10

Duration: 4 hours

Location: Institute for Immunology and Transfusion Medicine - Department Immunology (Sauerbruchstraße, Klinikum DZ7)



3. Pathogen driven evolution of the immune system

It is well accepted that pathogens adapt to their hosts due to a short generation time and a high mutation rate. Whether host adaption to pathogens occurs, however, is still under debate. In this workshop basic mechanisms of host pathogen coevolution will be presented and evidence that support pathogen driven host evolution will be discussed. Using bioinformatic tools participants of the workshop will analyze the evolution of a selected immune receptor gene family and determine whether individual genes are under purifying selection or under selection for diversification. Results will be discussed in view of their relevance to support the existence of host-pathogen coevolution.


Realized by: FLI – Friedrich-Loeffler-Institut (PD Dr. Robert Kammerer)

Attendance: 10–16

Duration: will follow

Location: Computer Cabinet



4. Bacterial phagocytosis by human macrophages

Professional phagocytes are an important part of the immune system. Macrophages are responsible for the recognition and elimination of bacteria. After phagocytosis and lysosomal degradation, fragments of bacteria are presented to T-cells of the adaptive immune system. Therefore, macrophages present an important link between the innate and the adaptive immune system. In this seminar cell culture infection experiments with pneumococci and human monocytic THP-1 cells will be presented. Immunofluorescence microscopy with differentially stained intra- and extracellular bacteria located inside or outside of THP-1 cells will be carried out.

Practical work/demonstration, for all students. The workshop consists of two parts:


4.1 Nasopharyngeal and bronchoalveolar lavage – Isolation of bacteria from infected mice

Besides in vitro experiments, like cell cultures, in vivo assays are a very important approach to analyze bacteria/host interactions in a complex living system. Pneumococcal colonization of the nasopharynx and the infection of the lung can be analyzed in different mouse models. After intranasal infection of mice with bioluminescent Streptococcus pneumoniae, pneumococcal dissemination can be monitored using a bio-imaging system. Another possibility is the determination of the colony forming units isolated from different organs of infected animals.

After a brief presentation about different mouse models, used in the group of Prof. Hammerschmidt, nasopharyngeal and bronchoalveolar lavage in mice will be demonstrated.

Seminar/demonstration, for all students.


4.2 Transfection of eukaryotic HEK293T-cells – GFP expression

The transfer of RNA or DNA into eukaryotic cells is a widely-used procedure to inhibit the expression of specific genes (siRNA), to study and control gene expression or to heterologously express proteins in cell cultures. Various methods for transfection of eukaryotic cells are available. Here, we present the calcium phosphate transfection method with a plasmid containing a gene encoding for the green fluorescence protein (GFP). After a brief overview of transfection methods and applications, transfected HEK-293T cells will be analyzed using fluorescence microscopy.

Practical work/demonstration, for all students


Realized by: Institute for Genetics and functional Genomics (Dr. Thomas Kohler)

Attendance: 12

Duration: will follow

Location: will follow



5. Protein separation and identification in the field of infection biology

The identification and quantitation of proteins from pathogen bacteria play an important role in understanding the pathophysiology of bacteria in simulated interaction studies with e.g. human cell lines. Because of the high cellular complexity in host-pathogen interaction samples, it is very difficult to measure both the pathogen- and the hostproteins simultaneously in one mass spectrometry run. Pre-fractionation by e.g. cell sorting or 1D PAGE is the key aspect to overcome such fundamental problems. How we apply such techniques can be examined by the students.


Realized by: Institute for Genetics and functional Genomics (Dr. Dipl.-Ing. Frank Schmidt)

Attendance: 12

Duration: will follow

Location: will follow



6. Mass spectrometric identification of proteins – sample preparation and data analysis

This workshop will convey how proteins are identified from an SDS-PAGE gel or a dissolution by mass spectrometry. After a quick theoretical introduction about mass spectrometric methods, the students will learn in a practical part how to prepare proteins from an SDS-PAGE gel and how to prepare the sample for the mass spectrometric analysis. Afterwards, the participants will have the opportunity to experience different types of mass spectrometers in action.


Realized by: Institute for Mikrobiology (Sandra Maaß)

Attendance: 6

Duration: will follow

Location: will follow



7. Digital recording and editing in microscopy

Picture recording using a digital camera often is the first step in creating a meaningful microscopic picture. The subsequent editing achieves two important functions
i) Pixel data of the recording have to correlate with the true light intensity of the object
ii) it is an important tool to create figures for scientific publications.
Therefore, the edited picture needs to reflect the conditions of the objects as objectively as possible. During this workshop, we will discuss fundamental methods of digital image editing and work out guidelines for creating figures, which can be part of scientific publications.


Realized by: Institute for Mikrobiology (Dr. Jan Pané Farré)

Attendance: 12

Duration: will follow

Location: Institute for Mikrobiology (Friedrich-Ludwig-Jahn-Straße 15)



8. Genotyping of ACE-Allels

Genotyping is a commonly used method in science and medicine. It is an easy way to determine fatherhood/motherhood, the genotype of transgenic animals, but also mutations that lead or might lead to certain diseases.

The angiotensine converting enzyme (ACE) is an enzyme that converts angiotensine I into angiotensine II, which is a very potent vasoconstrictor. It has been shown that 2 genetic variants encode the ACE-gen which differ in their length. This polymorphism has no influence on the enzymes structure but might be important for the regulation of this gene. Clinical studies showed a higher risk for cardiovascular diseases, diabetic induced long term damage, and development of renal complications due to underlying diseases for one possible genotype of the ACE-gen.

In this workshop, after a short introduction, students will get the opportunity to analyse and genotype DNA. Therefore each student will isolate genomic DNA from own blood samples (not necessarily) and perform a polymerase chain reaction. The results will be discussed in view of clinical relevance.


Realized by: Institute for Medical Biochemistry and Molecular Biology (Manuela Gellert)

Attendance: 15

Duration: will follow

Location: Institute for Medical Biochemistry and Molecular Biology (Sauerbruchstraße, Klinikum DZ7)



9. Use of metabolom analysis of urin – Introduction to the software R

The workshop will give a practical introduction into the software R for the statistical evaluation of data. The data will be collected by Nuclear magnetic resonance spectroscopy. Thereby, univariate test procedures (Student`s t-test or Wilcoxon rank-sum test) as well as multivariate procedures (Principle Component Analysis) will find use. Furthermore, we will place importance on the graphical workup of the results. The participants should already be familiar with basic statistical procedures.


Realized by: Institute for Clinical Chemistry and Laborytory Medicine (Maik Pietzner)

Attendance: 10-15

Duration: will follow

Location: Computer Cabinet with R-Software



10. RNA as drug and vaccine

Over the past decade nucleic acids have been increasingly considered for therapeutic applications. Antisense RNA, DNAzymes, small interfering RNAs (siRNAs) and trans-cleaving ribozymes are utilized to inhibit undesired gene expression of therapeutically relevant targets. Furthermore, several strategies focusing on mRNA repair have been evolved to address the challenging task of correcting genetic disorders. Lastly, also mRNA-based vaccination strategies have emerged as alternative to classic peptide based vaccines. In the seminar, basics of RNA as drug and vaccine as well as recent progress in the field will be discussed. Prof. Müller will also offer a guided tour through the department with special focus on RNA synthesis facilities.


Realized by: Institute for Biochemistry (Prof. Sabine Müller)

Attendance: 20

Duration: will follow

Location: Institute of Biochemistry (Felix-Hausdorff-Straße 4)



11. Introduction into basic histology to study animal tissues

The preparation of histological thin sections of paraffin-embedded tissues and their histological staining is a standard technique in microscopic diagnostic and research of animal tissues. During this workshop the basics of this technique such as fixation, preparation and embedding of tissue samples will be conveyed. Furthermore, the preparation of sections using a microtome will be demonstrated. The participants will have the opportunity to stain their own prepared sections (HE stain) and to convert them into permanent histological specimen.
Please bring along a lab coat to protect your clothes from the dyestuff.


Realized by: Institute for Cytology and Evolutionary Biology (Prof. Steffen Harzsch)

Attendance: 10

Duration: will follow

Location: Institute for Cytology and Evolutionary Biology (Soldmannstraße 23)



12. Flow cytometric analyses of platelet activation

In whole blood or blood products like platelet concentrates (PC) the measurement of platelet (pre-) activation by different agonists is an important marker for indication of the platelet status. Besides functional studies by aggregometry, flow cytometry is used as a fast and reliable method for high throughput analysis of activation markers like P-selectin. The workshop addresses the basics in flow cytometry and how to plan experiments with whole blood or purified platelets. Each student will have the opportunity to activate and stain their own samples, create and adjust flow cytometer protocols to measure the P-selectin and PAC-1 expression.


Realized by: Institute for Immunology and Transfusion Medicine - Department of Transfusion Medicine (Jan Wesche)

Attendance: 6-12

Duration: will follow

Location: Institute for Immunology and Transfusion Medicine - Department of Transfusion Medicine (Sauerbruchstraße, DZ7, J04.34)


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